توضیحات محصول
Sample Stability
Samples are stable up to 1 week at +2 to +8 °C. For longer storage periods keep samples frozen below -18 °C.
Injection Volume
10 to 50 μl
Flow
Pump A: 0.02–1.00 ml/min
Pump B: 0.60–1.00 ml/min
Additional Info
HPLC parameters
Pump A delivers 100 % Mobile Phase A for loading the trap column.
Pump B delivers 100 % Mobile Phase A for separating analytes on the analytical column.
Ionisation
APCI positive
MS/MS-Mode
MRM
Method of Analysis
LC-MS/MS
Parameter
25-OH-vitamin D2, 25-OH-vitamin D3
Analysis Time
5 min
Limit of quantification
25-OH-Vitamin D2: 2.2 µg/l
25-OH-Vitamin D3: 3.0 µg/l
Linearity
up to 250 μg/l
Intraassay
CV < 5 %
Interassay
CV < 6 %
Information
The trap column and the analytical column are linked to the mass spectrometer through a 2-position 6-port valve.
Specimen
Serum/Plasma
Pre-analytic Treatment
Patient samples are stable up to 3 days at ambient temperature and up to 1 week at +2 to +8 °C. For longer storage (maximum 1 month) deep-freeze the samples below -18 °C.
سایر محصولات مرتبط