توضیحات محصول
Specimen
Serum/Plasma
Sample Preparation
- Put Sample Clean Up Columns into tubes for sample preparation.
- Pipette 0.8 ml Equilibration Reagent 1 into each Sample Clean Up Column, centrifuge and repeat with Equilibration Reagent 2.
- Pipette 500 μl sample/calibrator/MassCheck® control, 50 μl Internal Standard Mix and 450 μl Extraction Buffer into each Sample Clean Up Column, mix briefly (vortex) and centrifuge.
- Add 2 x 0.7 ml Wash Buffer and centrifuge.
- Put autosampler vials into tubes for sample preparation, then put Sample Clean Up Columns on top.
- Add 1 ml Elution Buffer and centrifuge.
- Evaporate eluates in the autosampler vials (nitrogen or compressed air) to dryness.
- Add 100 μl Reconstitution Buffer.
- Close autosampler vials, mix briefly (vortex) and inject 5–50 μl of the eluate.
Sample Stability
The stability depends on the specific steroid. Further information can be obtained from the instruction manual.
Injection Volume
5-50 µl
Ionisation
ESI negative and positive (ESI switch)
MS/MS-Mode
MRM
- 11-Deoxycorticosterone
- 11-Deoxycortisol
- 17-OH-progesterone
- 21-Deoxycortisol
- Aldosterone
- Androstenedione
- Corticosterone
- Cortisol
- Cortisone
- Dehydroepiandrosterone (DHEA)
- Dehydroepiandrosterone sulfate (DHEA-S)
- Dihydrotestosterone (DHT)
- Estradiol
- Progesterone
- Testosterone
Method of Analysis
LC-MS/MS
Number of Tests
96
Parameter
11-Deoxycorticosterone, 11-Deoxycortisol, 17-OH-progesterone, 21-Deoxycortisol, Aldosterone, Androstenedione, Corticosterone, Cortisol, Cortisone, Dehydroepiandrosterone (DHEA), Dehydroepiandrosterone sulfate (DHEA-S), Dihydrotestosterone (DHT), Estradiol, Progesterone, Testosterone
Analysis Time
Steroid Panel 1: 10.0 min; Steroid Panel 2: 11.7 min
Limit of quantification
Steroid Panel 1: down to 14 ng/l
Steroid Panel 2: down to 5 ng/l
Linearity
Steroid Panel 1: covering reference range of each steroid
Steroid Panel 2: covering reference range of each steroid
Recovery
Steroid Panel 1: 86-115 %; Steroid Panel 2: 76-118 %
Intraassay
Steroid Panel 1: CV 1-6 %
Steroid Panel 2: CV 1-13 %
Interassay
Steroid Panel 1: CV 3-11 %
Steroid Panel 2: CV 4-13 %
Gradient
binary
Column temperature
Steroid Panel 1: 32 °C, Steroid Panel 2: 27 °C
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