توضیحات محصول
Specimen
Saliva
Sample Preparation
- Pipette 100 μl sample/calibrator/MassCheck® control into a Clean-Up Tube.
- Add 50 μl Internal Standard Mix.
- Centrifuge 15 min at 14000 x g.
- Transfer each filtrate into a 1.5 ml vial equipped with micro-inserts.
- Seal each vial and transfer into autosampler.
- Inject up to 30 μl.
Injection Volume
≤ 30 µl
Ionisation
ESI positive
MS/MS-Mode
MRM
Method of Analysis
LC-MS/MS
Number of Tests
400
Parameter
Cortisol, Cortisone
Analysis Time
4.6 min
Limit of quantification
0.3 µg/l
Linearity
up to 120 μg/l (Cortisol)
up to 180 μg/l (Cortisone)
Recovery
96–105 %
Intraassay
CV = 2–5 %
Interassay
CV = 2–7 %
Gradient
binary
Column temperature
ambient (~ 25 °C)
سایر محصولات مرتبط