توضیحات محصول
Specimen
Serum/Plasma
Sample Preparation
- Put 96 SPE Well Plate onto Waste Plate.
- Pipette 0.8 ml Equilibration Reagent 1 into each well of the 96 SPE Well Plate, centrifuge and repeat with Equilibration Reagent 2.
- Pipette 500 μl sample/calibrator/MassCheck® control, 50 μl Internal Standard Mix and 450 μl Extraction Buffer into each well, mix briefly (vortex) and centrifuge.
- Add 2 x 0.7 ml Wash Buffer and centrifuge.
- Place plate onto the Collection Plate.
- Add 500 μl Elution Buffer and centrifuge.
- Evaporate eluates in the Collection Plate (nitrogen or compressed air) to dryness.
- Add 100 μl Reconstitution Buffer.
- Shake Collection Plate, seal with adhesive seal and inject 5–50 μl of the eluate.
Sample Stability
The stability depends on the specific steroid. Further information can be obtained from the instruction manual.
Injection Volume
5-50 µl
Ionisation
ESI negative and positive (ESI switch)
MS/MS-Mode
MRM
- 11-Deoxycorticosterone
- 11-Deoxycortisol
- 17-OH-progesterone
- 21-Deoxycortisol
- Aldosterone
- Androstenedione
- Corticosterone
- Cortisol
- Cortisone
- Dehydroepiandrosterone (DHEA)
- Dehydroepiandrosterone sulfate (DHEA-S)
- Dihydrotestosterone (DHT)
- Estradiol
- Progesterone
- Testosterone
Method of Analysis
LC-MS/MS
Parameter
11-Deoxycorticosterone, 11-Deoxycortisol, 17-OH-progesterone, 21-Deoxycortisol, Aldosterone, Androstenedione, Corticosterone, Cortisol, Cortisone, Dehydroepiandrosterone (DHEA), Dehydroepiandrosterone sulfate (DHEA-S), Dihydrotestosterone (DHT), Estradiol, Progesterone, Testosterone
Analysis Time
Steroid Panel 1: 10.0 min; Steroid Panel 2: 11.7 min
Limit of quantification
Steroid Panel 1: down to 14 ng/l
Steroid Panel 2: down to 5 ng/l
Linearity
Steroid Panel 1: covering reference range of each steroid
Steroid Panel 2: covering reference range of each steroid
Recovery
Steroid Panel 1: 86-115 %; Steroid Panel 2: 76-118 %
Intraassay
Steroid Panel 1: CV 1-6 %
Steroid Panel 2: CV 1-13 %
Interassay
Steroid Panel 1: CV 3-11 %
Steroid Panel 2: CV 4-13 %
Gradient
binary
Column temperature
Steroid Panel 1: 32 °C, Steroid Panel 2: 27 °C
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