توضیحات محصول
Column temperature
ambient temperature ( ~ 25 °C)
Flow rate
1.5 ml/min
Specimen
Whole blood
Sample Preparation
Dilute 5 to 8 µl whole blood with 1 ml Hemolysis Reagent, mix well.
Inject 10 to 20 µl of the hemolysis mixture into the HPLC system.
Samples prepared as above can be stored at ambient temperature for about 24 hours, at +2 to +8 °C up to 36 h, frozen below -18 °C up to 3 months.
Sample Stability
At ambient temperature approx. 3 days, at +2 to +8 °C up to 1 week and below -18 °C up to 6 months. Once thawed, samples should not be refrozen.
Injection Volume
10-20 µl
Additional Info
For the analysis of hemoglobin variants any HPLC gradient system with UV/VIS detector is suitable.
Wavelength
415 nm
Method of Analysis
HPLC
Number of Tests
1000
Parameter
HbA, HbA1c, HbA2, HbC, HbD, HbE, HbF, HbS
Analysis Time
9 min
Limit of quantification
HbA2: 2 %
HbF: 1 %
Linearity
HbA2: up to at least 75 %
HbF: up to at least 70 %
Recovery
HbA2: 100 %; HbF: 99.2 %
Intraassay
CV < 8 %
Interassay
CV < 4 %
سایر محصولات مرتبط