MassChrom® Steroids in Serum/Plasma with Sample Clean Up Columns – LC-MS/MS


شماره سفارش : 72072/C

تست‌ های قابل اجرا با هر کیت : 96

روش بهینه و گسترده

آنالیز 15 استروئید مختلف

دارای ستون Sample Clean Up

Method of Analysis

LC-MS/MS

Number of Tests

96

Parameter

11-Deoxycorticosterone, 11-Deoxycortisol, 17-OH-progesterone, 21-Deoxycortisol, Aldosterone, Androstenedione, Corticosterone, Cortisol, Cortisone, Dehydroepiandrosterone (DHEA), Dehydroepiandrosterone sulfate (DHEA-S), Dihydrotestosterone (DHT), Estradiol, Progesterone, Testosterone

Analysis Time

Steroid Panel 1: 10.0 min; Steroid Panel 2: 11.7 min

Limit of quantification

Steroid Panel 1: down to 14 ng/l
Steroid Panel 2: down to 5 ng/l

Linearity

Steroid Panel 1: covering reference range of each steroid
Steroid Panel 2: covering reference range of each steroid

Recovery

Steroid Panel 1: 86-115 %; Steroid Panel 2: 76-118 %

Intraassay

Steroid Panel 1: CV 1-6 %
Steroid Panel 2: CV 1-13 %

Interassay

Steroid Panel 1: CV 3-11 %
Steroid Panel 2: CV 4-13 %

Gradient

binary

Column temperature

Steroid Panel 1: 32 °C, Steroid Panel 2: 27 °C

Specimen

Serum/Plasma

Sample Preparation

  • Put Sample Clean Up Columns into tubes for sample preparation.
  • Pipette 0.8 ml Equilibration Reagent 1 into each Sample Clean Up Column, centrifuge and repeat with Equilibration Reagent 2.
  • Pipette 500 μl sample/calibrator/MassCheck® control, 50 μl Internal Standard Mix and 450 μl Extraction Buffer into each Sample Clean Up Column, mix briefly (vortex) and centrifuge.
  • Add 2 x 0.7 ml Wash Buffer and centrifuge.
  • Put autosampler vials into tubes for sample preparation, then put Sample Clean Up Columns on top.
  • Add 1 ml Elution Buffer and centrifuge.
  • Evaporate eluates in the autosampler vials (nitrogen or compressed air) to dryness.
  • Add 100 μl Reconstitution Buffer.
  • Close autosampler vials, mix briefly (vortex) and inject 5–50 μl of the eluate.

Sample Stability

The stability depends on the specific steroid. Further information can be obtained from the instruction manual.

Injection Volume

5-50 µl

Ionisation

ESI negative and positive (ESI switch)

MS/MS-Mode

MRM


  • 11-Deoxycorticosterone
  • 11-Deoxycortisol
  • 17-OH-progesterone
  • 21-Deoxycortisol
  • Aldosterone
  • Androstenedione
  • Corticosterone
  • Cortisol
  • Cortisone
  • Dehydroepiandrosterone (DHEA)
  • Dehydroepiandrosterone sulfate (DHEA-S)
  • Dihydrotestosterone (DHT)
  • Estradiol
  • Progesterone
  • Testosterone

سایر محصولات

اسپکتروفتومتر UV-1900 شیمادزو

ترازوهای آزمایشگاهی سری TW/TWC
برنامه‌ی توزین
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تکنولوژی UniBloc
ترازوهای آزمایشگاهی
ایکس ری
توزیع اندازه‌ی ذرات
آنالایزر حرارتی
DSC-60 Plus
IRSpirit