MassChrom® Steroids in Serum/Plasma with 96-SPE-Well-Plate – LC-MS/MS


شماره سفارش : 72072/96, 72072/480

تست‌ های قابل اجرا با هر کیت : 96 / 480

روش بهینه و گسترده

آنالیز 15 استروئید مختلف

دارای 96 SPE Well Plate

Method of Analysis

LC-MS/MS

Parameter

11-Deoxycorticosterone, 11-Deoxycortisol, 17-OH-progesterone, 21-Deoxycortisol, Aldosterone, Androstenedione, Corticosterone, Cortisol, Cortisone, Dehydroepiandrosterone (DHEA), Dehydroepiandrosterone sulfate (DHEA-S), Dihydrotestosterone (DHT), Estradiol, Progesterone, Testosterone

Analysis Time

Steroid Panel 1: 10.0 min; Steroid Panel 2: 11.7 min

Limit of quantification

Steroid Panel 1: down to 14 ng/l
Steroid Panel 2: down to 5 ng/l

Linearity

Steroid Panel 1: covering reference range of each steroid

Steroid Panel 2: covering reference range of each steroid

Recovery

Steroid Panel 1: 86-115 %; Steroid Panel 2: 76-118 %

Intraassay

Steroid Panel 1: CV 1-6 %
Steroid Panel 2: CV 1-13 %

Interassay

Steroid Panel 1: CV 3-11 %
Steroid Panel 2: CV 4-13 %

Gradient

binary

Column temperature

Steroid Panel 1: 32 °C, Steroid Panel 2: 27 °C

Specimen

Serum/Plasma

Sample Preparation

  • Put 96 SPE Well Plate onto Waste Plate.
  • Pipette 0.8 ml Equilibration Reagent 1 into each well of the 96 SPE Well Plate, centrifuge and repeat with Equilibration Reagent 2.
  • Pipette 500 μl sample/calibrator/MassCheck® control, 50 μl Internal Standard Mix and 450 μl Extraction Buffer into each well, mix briefly (vortex) and centrifuge.
  • Add 2 x 0.7 ml Wash Buffer and centrifuge.
  • Place plate onto the Collection Plate.
  • Add 500 μl Elution Buffer and centrifuge.
  • Evaporate eluates in the Collection Plate (nitrogen or compressed air) to dryness.
  • Add 100 μl Reconstitution Buffer.
  • Shake Collection Plate, seal with adhesive seal and inject 5–50 μl of the eluate.

Sample Stability

The stability depends on the specific steroid. Further information can be obtained from the instruction manual.

Injection Volume

5-50 µl

Ionisation

ESI negative and positive (ESI switch)

MS/MS-Mode

MRM


  • 11-Deoxycorticosterone
  • 11-Deoxycortisol
  • 17-OH-progesterone
  • 21-Deoxycortisol
  • Aldosterone
  • Androstenedione
  • Corticosterone
  • Cortisol
  • Cortisone
  • Dehydroepiandrosterone (DHEA)
  • Dehydroepiandrosterone sulfate (DHEA-S)
  • Dihydrotestosterone (DHT)
  • Estradiol
  • Progesterone
  • Testosterone

سایر محصولات

اسپکتروفتومتر UV-1900 شیمادزو

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آنالایزر حرارتی
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IRSpirit